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鉴于有抗Ro/SSA抗体的新生儿SLE、SCLE及“ANA阴性”SLE患者易发生光暴露部位的皮损,以及新生儿LE中Ro/SSA抗体似与皮疹及可能的心脏受累有关,作者检查了胎儿,新生儿及成人皮肤和胎儿心脏中的Ro/SSA抗源,并进行了分析比较。用含单一特异的抗Ro/SSA抗体的人血清,分别以胎儿(12,18及24周龄)、新生儿及成人皮肤为底物,进行间接免疫荧光检测,发现胎儿及新生儿皮肤角朊细胞核中有荧光染色,而成人角朊细胞为阴性,如以体外培养的成人皮片为底物进行检测,则角朊细胞中有荧光染色。Ro/SSA抗原在角朊细胞中呈现大的斑点样线状型荧光染色。用同样的方法,以胎儿心脏为底物,进行检测,虽然证阴有Ro/SSA抗原在心肌细胞中存在,但定位难以确定,可能存在于细胞核及细胞膜上。进一步从胎儿皮肤
In view of neonatal SLE with anti-Ro / SSA antibodies, skin lesions at sites of light exposure prone to SCLE and “ANA-negative” SLE, and Ro / SSA antibodies in neonatal LE appear to be associated with rash and possible cardiac involvement, the authors examined Ro / SSA sources in fetal, neonatal and adult skin and fetal heart were analyzed and compared. Indirect immunofluorescence assay using fetal serum (12, 18 and 24 weeks old), neonatal and adult skin as substrate, respectively, with human serum containing a single specific anti-Ro / SSA antibody revealed that fetal and neonatal keratin Fluorescent staining in the nucleus, while adult keratinocytes are negative, as in vitro culture of adult skin as a substrate for detection, the keratinocytes in a fluorescent staining. Ro / SSA antigen presents a large spot-like linear fluorescent staining in keratinocytes. In the same way, the fetus heart as a substrate for testing, although evidence of Ro / SSA antigen in the presence of myocardial cells, but the positioning is difficult to determine, may exist in the nucleus and the cell membrane. Further from the fetus skin