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目的研究经大鼠尾静脉注入不同剂量鞭毛蛋白后,观察不同时相点急性肺损伤(acute lung injury,ALI)发生的情况,探讨其发生的可能机制。方法240只清洁级雄性Wistar大鼠分为4组:对照组、致伤1组、致伤2组和致伤3组。对照组给予生理盐水,各致伤组分别给予5、50、500μg/kg的鞭毛蛋白,于静注后2、4、6、12、24、48h,测定动脉血氧分压(PaO2)、肺组织湿干比值(W/D)、血清与支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α(TNF-α)和白介素-lβ(IL-lβ)细胞因子的含量以及观察肺组织的病理改变。结果静注鞭毛蛋白后,在对应时相点,鞭毛蛋白注入剂量越大,大鼠PaO2越低,肺W/D越高;外周血及BALF中,细胞因子TNF-α、IL-lβ含量越高;肺组织病理改变越明显。以上各指标在各对应时相点,致伤2、3组与对照组比较差异有统计学意义(P<0.01);致伤2、3组与致伤1组间也有显著性差异(P<0.01);致伤2组与致伤3组间仅TNF-α含量比较差异有统计学意义(P<0.01)。结论鞭毛蛋白可致大鼠急性肺损伤,且其具有剂量和时间的差异性。
Objective To investigate the occurrence of acute lung injury (ALI) at different time points after injection of different doses of flagellin into rat caudal vein and to explore the possible mechanism. Methods Two hundred and forty clean male Wistar rats were divided into 4 groups: control group, injured group 1, injured group 2 and injured group 3. The control group was given normal saline, and each injury group was given 5, 50 and 500μg / kg of flagellin respectively. After 2, 4, 6, 12, 24 and 48 hours after intravenous injection, PaO2, (W / D), the contents of TNF-α and IL-1β in serum and bronchoalveolar lavage fluid (BALF) and the pathological changes of lung tissue . Results After intravenous injection of flagellin, the higher the dose of flagellin was, the lower the PaO2 was and the higher the W / D of lung was at the corresponding time points. The contents of TNF-α and IL-1β in peripheral blood and BALF were higher High; the more obvious pathological changes of lung tissue. There was a significant difference between the two groups (P <0.01). There was also a significant difference between the two groups (P < 0.01). There was significant difference in the level of TNF-αin the two groups (P <0.01). Conclusion Flagellin can cause acute lung injury in rats, and it has the dose and time difference.