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目的 探讨反义胰岛素样生长因子Ⅱ (反义IGF -Ⅱ )对人肝癌细胞株HepG2 恶性表型的抑制。方法 化学合成人IGF -Ⅱ 5’端部分cDNA片段 ,将其克隆于真核表达载体pcDNA3 ,通过脂质体介导转入体外培养的HepG2 人肝癌细胞 ,观察细胞生物学特性的改变。结果 将反义IGF -Ⅱ转导入HepG2 肝癌细胞后 ,细胞内IGF -Ⅱ表达量减少 (荧光强度由 19.9± 3.1降至 6 .2± 1.8,(P <0 .0 1) ,细胞增殖率下降、凋亡率增加 (由 4 .3%± 0 .5 0 %增加至 8.6 %± 0 .5 0 % ,(P <0 .0 1)、AFP分泌水平降低 (由5 .4 7μg/l± 0 .5 6 μg/l降至 1.95 μg/l± 0 .10 μg/l,(P <0 .0 1)。 结论 反义IGF -Ⅱ可显著抑制人肝癌细胞恶性表型 ,对于治疗原发性肝癌可能具有潜在应用价值。
Objective To investigate the inhibitory effect of antisense insulin-like growth factor Ⅱ (antisense IGF-Ⅱ) on the malignant phenotype of human hepatocellular carcinoma cell line HepG2. Methods The human 5’-end cDNA fragment of human IGF-Ⅱ was chemically synthesized and cloned into the eukaryotic expression vector pcDNA3. The recombinant plasmid was transfected into HepG2 human hepatocellular carcinoma cells by liposome and the changes of cell biological characteristics were observed. Results After transfection of antisense IGF-Ⅱ into HepG2 hepatoma cells, the expression of IGF-Ⅱ decreased (the fluorescence intensity decreased from 19.9 ± 3.1 to 6.2 ± 1.8, P <0.01), and the cell proliferation rate decreased , The apoptotic rate increased from 4.3% ± 0.05% to 8.6% ± 0.55% (P <0.01), and the AFP secretion decreased (from 5.47 μg / 1 ± 0.56 μg / l to 1.95 μg / l ± 0.10 μg / l, respectively (P <0.01) .Conclusion Antisense IGF-Ⅱ can significantly inhibit the malignant phenotype of human hepatocellular carcinoma cells, HCC may have potential value.