Cloning, Sequencing and Expression in E. coli of Interferon-ω1 Gene

• 作 者: 黎孟枫 曾庆 周园 郭辉玉 侯云德
• 机 构: InstituteofVirology,ChineseAcademyofPreventiveMedicine,NationalLaboratoryofMolecularVirology,Beijing
• 出 处: Science in China,Ser.B
• 发表日期: 1993年11期
• 关键词: Cloning Sequencing Gene cloned promoter codon homology practically considerably

Human interferon ω1 （huIFN-ω1） gene was isolated and cloned from chromosome DNA derived from a Chinese fetal liver via polymerase chain reaction （PCR）. By determining its nucleotide sequence we proved that the 88th codon should be GGA, coding for Gly. Aft