论文部分内容阅读
柑桔属的大多数栽培种和品种的种子存在珠心胚的多胚现象,它严重干扰了柑桔的杂交育种工作。本文报道在双目解剖镜下刚能看到幼胚时就进行幼胚的离体培养,可能是避过了多胚的干扰的办法之一。研究的材料是暗柳甜橙(Citrus sinensis Osbeck)和椪柑(Citrusreticulata Blanco)。取暗柳甜橙开花后71天和椪柑开花后90天的直径为0.04—0.10毫米的幼胚于试管中培养。以Marashige和Tucker培养基为基本培养基,附加蜂乳(RJ)400ppm和赤微酸(GA)1ppm。经一个多星期的培养,幼胚已开始长成为较大的子叶胚。以后长成小苗为接种胚数的58.82％(甜橙)和55.0％(椪柑)。小苗长到2—3厘米高时,转移到含有萘乙酸(NAA)1ppm的MT培养基,大部分都能长出根系 试管小苗长至3—4厘米高时,嫁接在盆栽砧木上(红(木黎)檬或其他砧木实生苗),成活率达85.7％。未经诱导生根的小苗同样能嫁接成功。接活后小苗生长快,4个月苗高30厘米,可以移栽于露地。
The majority of citrus cultivars and varieties of seed exist bead embryo polyembryon phenomenon, it seriously disrupted the cross-breeding of citrus. This paper reports that the in vitro culture of immature embryos can be seen just when the embryos are seen in the binocular dissection microscope, which may be one of the ways to avoid the interference of polyembryo. The materials studied were Citrus sinensis Osbeck and Citrus reticulata Blanco. The immature embryos with diameter of 0.04-0.10 mm were cultured in vitro after 71 days of flowering dark orange and orange blossom and 90 days after flowering of citrus fruit. Marashige and Tucker media were used as basal medium supplemented with 400 ppm of royal jelly (RJ) and 1 ppm of trichosinic acid (GA). After more than a week of culture, immature embryos have begun to grow into larger cotyledons. After growing into seedlings for the number of embryos inoculated 58.82% (sweet orange) and 55.0% (citrus). When young shoots grow to 2-3 cm in height, they are transferred to MT medium containing 1 ppm of NAA, most of which can grow in vitro. The shoots grow to 3-4 cm in height and are grafted onto potted rootstocks (red ( Muli) lemon or other rootstock seedlings), the survival rate of 85.7%. Seedlings without rooting can also be grafted successfully. After the live seedlings grow fast, 4 months seedling height 30 cm, you can transplanted in the open field.