论文部分内容阅读
目的比较结香与未结香白木香内生细菌群落结构及变化,探讨内生细菌在白木香结香过程中的作用。方法分别提取结香与未结香白木香总DNA,采用细菌16s r DNA通用引物进行扩增,聚合酶链反应-变性梯度凝胶电泳(Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis,PCR-DGGE)比较各样品内生细菌群落结构差异,结合样品薄层色谱(TLC)分析,研究活性成分与内生细菌的关联。结果多食鞘氨醇杆菌Sphingobacterium multivorum在未结香白木香中普遍存在。结香后,内生细菌丰富度和多样性明显增加,PCR-DGGE图谱聚类表明,绝大部分结香样品细菌群态相似性较高,聚在一起;非结香样品间的细菌群态相似性也较高。结论白木香结香前后内生细菌群落存在明显差异,内生细菌可能参与白木香的结香过程。
OBJECTIVE To compare the endophytic bacteria community structure and changes in the fruiting bodies and unmerged white woods, and to explore the role of endophytic bacteria in the process of white woody flowers. Methods Total DNA was extracted from Fructus Gordianus and Fructus uncinus, amplified with bacterial 16s r DNA universal primers, and compared with PCR-DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis) The differences of endophytic bacteria communities in each sample were studied. The correlation between the active components and endophytic bacteria was studied by TLC analysis. As a result, Sphingobacterium multivorum was more commonly found in unmarked woody woody plants. Results showed that the endophytic bacteria richness and diversity were significantly increased after PCR-DGGE analysis. The cluster analysis by PCR-DGGE indicated that the majority of the samples were similar and clustered together. The bacterial community Similarity is higher. Conclusion The endophytic bacteria community existed obvious difference before and after the end of the plantation. The endophytic bacteria may participate in the process of the end of the plantation.