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目的:优化同时测定逍遥丸中芍药苷和槲皮素含量的方法。方法:采用HPLC法和菲罗门Luna-C18柱(250mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸,梯度洗脱;柱温:30℃,芍药苷、槲皮素的检测波长分别为230nm、254nm。流速:1.0mL.min-1(0～30min),0.8mL.min-1(30～60min)。结果:芍药苷和槲皮素的分离度很好,无其他杂质干扰,芍药苷在7.03～70.26μg/mL(r=0.999 7)、槲皮素在2.52～25.23μg/mL(r=0.999 7)范围内线性关系良好,平均加样回收率(n=6)分别为99.14%、98.69%,RSD分别为1.43%、1.40%。结论:该方法操作简便,结果准确,可用于逍遥丸中芍药苷与槲皮素的同时测定,为提高逍遥丸的质量标准提供了依据。
Objective: To optimize the method of simultaneous determination of paeoniflorin and quercetin in Xiaoyao Wan. METHODS: The HPLC method and Lilium-C18 column (250 mm × 4.6 mm, 5 μm) were used in the HPLC method. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The column temperature was 30 ℃, the detection wavelength of paeoniflorin and quercetin Respectively 230nm, 254nm. Flow rates: 1.0 mL.min-1 (0-30 min), 0.8 mL.min-1 (30-60 min). Results: The separation of paeoniflorin and quercetin was very good without any interference of other impurities. The concentrations of paeoniflorin at 7.03 ~ 70.26μg / mL (r = 0.999 7) and quercetin at 2.52 ~ 25.23μg / mL (r = 0.999 7 ). The average recoveries (n = 6) were 99.14% and 98.69%, respectively, with RSDs of 1.43% and 1.40%, respectively. Conclusion: The method is simple, accurate and can be used for the simultaneous determination of paeoniflorin and quercetin in Xiaoyao Wan. It provides the basis for improving the quality standard of Xiaoyao Pill.