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为研究硫代反义ｂｃｌ－２ 脱氧寡核苷酸（ＡＮ－ Ｓ－ ＯＤＮｓ） 对单核白血病细胞系Ｕ９３７ 细胞株凋亡的影响，采用Ｎｏｒｔｈｅｒｎ ｂｌｏｔ、ＤＮＡ 电泳、电镜和流式细胞计数技术检测了单核白血病细胞系Ｕ９３７ 细胞株ｂｃｌ－ ２ 基因的表达和ＡＮ－ Ｓ－ ＯＤＮｓ 对Ｕ９３７ 细胞凋亡的影响。结果发现Ｕ９３７ 细胞有大量ｂｃｌ－ ２ 基因表达；适当浓度（３０μｍｏｌ／Ｌ）ＡＮ－ Ｓ－ ＯＤＮｓ 使Ｕ９３７ 细胞出现凋亡ＤＮＡ 电泳梯状条带，电镜下可见部分细胞核浆比例缩小，核膜周边有核小体，流式细胞计数显示ｂｃｌ－ ２ 蛋白表达降低，细胞增殖能力降低。说明适当浓度的ＡＮ－ Ｓ－ ＯＤＮｓ 可以通过大大降低ｂｃｌ－２ 的表达而促进Ｕ９３７ 细胞凋亡。
To investigate the effects of antisense antisense bcl-2 deoxyribonucleotides (AN-S-ODNs) on the apoptosis of monocytic leukemia cell line U937, Northern blot, DNA electrophoresis, electron microscopy and flow cytometry The expression of bcl-2 gene in monocytic leukemia cell line U937 and the effect of AN-S-ODNs on the apoptosis of U937 cells were studied. The results showed that a large amount of bcl-2 gene was expressed in U937 cells. Apoptotic DNA electrophoresis ladder was found in U937 cells with appropriate concentration (30μmol / L) of AN-S-ODNs. Nucleosomes, flow cytometry showed decreased bcl-2 protein expression, decreased cell proliferation. This indicated that AN- S-ODNs in proper concentration could promote the apoptosis of U937 cells by greatly reducing the expression of bcl-2.