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本文应用单克隆抗体OKT系列、IL—2活性检测及短寿命Ts功能测定技术,对20例舍格林综合征(Sjog ren’s syndrome,SS)患者末稍血T淋巴细胞亚群、PBL产生IL—2能力及抑制性T细胞的功能进行了研究。结果显示SS在发病期间OKT_3、OKT_4阳性细胞比率较正常对照组明显降低(P<0.001),OKT_3阳性细胞比率高于正常对照组(P<0.001)。PBL产生IL—2较正常对照组明显降低(P<0.001),但Ts功能和正常对照组无明显差异(P>0.05)。原发性SS(6例)和继发性SS(14例)PBL产生IL—2活性及Ts功能,两组SS之间无明显差异。结果提示T_H细胞数量及功能降低,T细胞亚群数量及功能上失衡与本病的发病相关。
In this paper, the monoclonal antibody OKT series, IL-2 activity detection and short-lived Ts function measurement technology were used to generate IL-2 in peripheral blood T-lymphocyte subsets and PBL in 20 patients with Sjogren’s syndrome (SS). The ability and the function of suppressor T cells were studied. The results showed that the ratio of OKT_3 and OKT_4 positive cells was significantly lower in SS during the onset than in the normal control group (P<0.001), and the ratio of OKT_3 positive cells was higher in SS than in the normal control group (P<0.001). The IL-2 produced by PBL was significantly lower than that of the normal control group (P<0.001), but there was no significant difference between the Ts function and the normal control group (P>0.05). In the primary SS (6 cases) and secondary SS (14 cases), PBL produced IL-2 activity and Ts function, there was no significant difference between the two groups of SS. The results suggest that the number and function of T_H cells are reduced, and the imbalance in the number and function of T cell subsets is related to the pathogenesis of this disease.