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【目的】建立玉米杂交种与亲本苗期叶片差异表达蛋白谱,探讨叶片大小杂种优势形成的分子机理。【方法】以玉米强优势杂交种Mo17/B73及其亲本发芽后第5天的第3片叶为材料,采用双向电泳技术(2-DE),结合MALDI TOF MS质谱技术,建立叶片细胞分裂和生长关键区域的差异表达蛋白质谱,并对差异表达蛋白进行质谱鉴定。【结果】在检测到的630个蛋白质点中,有52个蛋白质点在杂交种与亲本之间的表达差异达到显著水平,表现为单亲沉默(15个)、偏高亲(13个)、偏低亲(8个)、杂种上调(6个)、杂种下调(7个)和杂种特异表达模式(3个)。另外,还成功鉴定出了其中的28个差异表达蛋白质点,涉及到代谢、胁迫响应、糖酵解、转录调控、蛋白折叠和降解、三羧酸循环、细胞骨架、发育及未知蛋白质等9个功能类别。【结论】玉米杂交种与亲本在蛋白丰度上存在明显的差异,并且差异表达蛋白涉及到多个功能类别,可能与玉米叶片大小杂种优势的形成有关。
【Objective】 The purpose of this study was to establish the differentially expressed protein profiles of seedling leaves of maize hybrids and their parents and to explore the molecular mechanism of leaf heterosis. 【Method】 Mo17 / B73, a dominant hybrid of maize, and the third leaf of the fifth day after germination of maize were used as materials. Two-dimensional gel electrophoresis (2-DE) and MALDI TOF MS were used to establish the cell division and Differences in growth-critical regions expressed protein profiles, and mass spectrometry identification of differentially expressed proteins. 【Result】 Among the 630 detected protein spots, 52 protein spots showed significant differences in expression level between hybrids and their parents, which showed 15 single parents, 13 high parents, Low progeny (8), hybrids up-regulated (6), hybrids down-regulated (7) and hybrid-specific expression patterns (3). In addition, 28 differentially expressed protein spots were successfully identified, including 9 metabolic and stress responses, glycolysis, transcriptional regulation, protein folding and degradation, tricarboxylic acid cycle, cytoskeleton, development and unknown proteins Function category. 【Conclusion】 There are significant differences in protein abundance between maize hybrids and parents, and the differentially expressed proteins involved in multiple functional classes may be related to the formation of maize leaf size heterosis.