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Ambroise-Thomas等(1974)曾用丝虫成虫冰冻切片抗原的免疫荧光抗体试验(IFAT)进行丝虫病流行病学监测,国内用于诊断丝虫病也取得较好效果。我们于1985年4～11月在海南岛琼山县和广西田阳县等不同防治阶段的丝虫病地区进行了防治监测的观察。 一、材料和方法 (一)抗原制备 从感染马来丝虫的长爪沙鼠取出雌性成虫,洗净,在-20℃条件下作冰冻切片(4～5μm厚),固定,待干后,于-30℃冷藏备用。(二)试验血清 在班氏丝虫病区的海南岛琼山县十字路乡的4个村,采集微丝蚴血症者耳垂血166例(未治疗者),在广西田阳县凤马村采集居民耳垂血149人(占全村人口80％,防治后8年).每人取耳垂血3大滴约60μl,滴于滤纸上、晾
Ambroise-Thomas et al. (1974) used the immunofluorescence antibody test (IFAT) of filariasis adult worms to carry out the epidemiological surveillance of filariasis. The diagnosis of filariasis in China also achieved good results. From April to November 1985, we observed the prevention and control of filariasis in different control stages such as Qiongshan County in Hainan Island and Tianyang County in Guangxi. I. Materials and Methods (I) Antigen preparation Female adult worms were removed from the gerbils infected with Malayi worms, washed, frozen sectioned (4 to 5 μm thick) at -20 ° C, fixed, and allowed to dry, Frozen at -30 ℃ spare. (II) Test Serum 166 ear lobster blood samples of microfilaremia were collected from 4 villages in Crossroad Township, Qiongshan County, Hainan Island in Bancroftian Wilt Disease Area (untreated persons) and collected in Fengma Village, Tianyang County, Guangxi The residents of earlobe blood 149 people (accounting for 80% of the village population, 8 years after the prevention and treatment.) Each person took three large drops of earlobe blood about 60μl, dropped on the filter paper, air