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目的 探讨p15基因变异及其与脑胶质瘤的发生、恶性进展的关系。方法 利用PCR和PCR -based甲基化检测技术检测了5 6例脑胶质瘤中p15基因外显子 1缺失及 5′CPG岛甲基化情况。结果 43例高级别的脑胶质瘤中 ,14例发生了p15基因缺失( 3 2 6% ) ,而 13例低级别的脑胶质瘤中无一例发生p15基因缺失 ,差异具有显著性 (P <0 0 5 )。 1例低级别的脑胶质瘤、3例高级别的脑胶质瘤发生了p15基因 5′CPG岛甲基化。结论 p15基因异常可能参与脑胶质瘤的发生、恶性进展。基因纯合缺失是脑胶质瘤中p15基因失活的主要机制。
Objective To investigate the variation of p15 gene and its relationship with the occurrence and malignant progression of glioma. Methods The deletion of exon 1 and 5’CPG island methylation of p15 gene in 56 gliomas were detected by PCR and PCR-based methylation detection. Results Of the 43 high-grade gliomas, 14 had a loss of p15 gene (32.6%), whereas none of 13 low-grade gliomas had a loss of the p15 gene (P <0 0 5). 1 case of low-grade glioma, 3 cases of high-grade glioma occurred p15 gene 5’CPG island methylation. Conclusion The abnormal p15 gene may be involved in the occurrence and malignant progression of glioma. Gene homozygous deletion is the main mechanism of p15 gene inactivation in gliomas.