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本文用简化Ｔａｔｅｓ微核试验法检测了苯、甲苯和二甲苯对小鼠睾丸生殖细胞的诱变性，用不同浓度的苯（４．５ｇ／ｍ３，９．０ｇ／ｍ３）、甲苯（２．５ｇ／ｍ３，５．０ｇ／ｍ３和二甲苯（２．５ｇ／ｍ３，５．０ｇ／ｍ３）分别经呼吸道对小鼠染毒，连续３天。于染毒首日后第１５天杀鼠制片，测得微核率６．８‰，７．２‰，３．０‰，２．４‰，３．４‰、４．０‰。这表明苯和二甲苯（５．０ｇ／ｍ３组）所致早期精细胞微核率明显高于阴性对照组（１．６‰），有统计学意义（ｐ＜０．０１或Ｐ＜０．０５），可认为是雄性生殖细胞染色体断裂剂。根据开始染毒到杀鼠制片的间隔时间判断，苯和二甲苯主要作用于减数分裂前的Ｇ１或Ｓ期初级精母细胞。
In this study, the mutagenicity of benzene, toluene and xylene in testis germ cells of mice was tested by the simplified Tates micronucleus test. Different concentrations of benzene (4.5g / m3, 9.0g / m3) and toluene The mice were inoculated via the respiratory tract for 3 consecutive days with 5g / m3, 5.0g / m3 and xylene (2.5g / m3, 5.0g / m3) The measured micronuclei rates were 6.8 ‰, 7.2 ‰, 3.0 ‰, 2.4 ‰, 3.4 ‰ and 4.0 ‰, indicating that benzene and xylene (5.0 g / m3 group ) Was significantly higher than the negative control group (1.6 ‰), which was statistically significant (p <0.01 or P <0.05), can be considered as a male germ cell chromosome rupture agent. Benzene and xylene act primarily on primary spermatocytes G1 or S prior to meiosis, as judged by the interval between the start of administration and the killing of rats.