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采用小牛胸腺提取１α，２５（ＯＨ）２维生素Ｄ３受体，基于未标记的和氚标记的１α，２５（ＯＨ）２维生素Ｄ３对其高特异性和高亲和力的受体发生竞争结合作用，可用以建立血清中１α，２５（ＯＨ）２Ｄ３含量的放射受体测定法。本室用一种简便易行的方法自胸腺制备了１α，２５（ＯＨ）２Ｄ３受体［１］，经放射受体测定法分析，该法制备的刚刚出生小公牛胸腺受体，其用量与标记物量匹配时，Ｂｏ可达４８．８％。试用于放射受体分析，血清１α，２５（ＯＨ）２Ｄ３的测定范围在３．９～６４．５ｐｇ／管，灵敏度为３．９ｐｇ／管，且样品前处理无需高效液相色谱［２］。
Using calf thymus to extract 1α, 25 (OH) 2 vitamin D3 receptors, competitive binding to its highly specific and high affinity receptors based on unlabeled and tritiated 1α, 25 (OH) 2 vitamin D3, Radioreceptor assays that can be used to establish 1 [alpha], 25 (OH) 2D3 content in serum. The room with a simple and easy method from the thymus preparation of 1α, 25 (OH) 2D3 receptor [1], by radioactive receptor assay analysis, the method of the newly born trombus recipient of young bulls, the amount and When the amount of markers match, Bo up to 48.8%. For the radioreceptor assay, serum 1α, 25 (OH) 2D3 was assayed at 3.9-64.5 pg / tube with a sensitivity of 3.9 pg / tube and no sample prepration required for HPLC [2].