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目的：诱导异种移植“体液适应”，并对其机制加以研究．方法：运用多种免疫抑制方法处理受体兔，然后行大鼠→兔骨髓、胸腺联合移植，并通过细胞毒试验、直接和间接免疫荧光染色等方法检测大鼠组织、细胞在受体兔体内的嵌合性；同时，荧光标记的羊抗大鼠单个核细胞抗体直接大鼠体内注射，通过流式细胞仪对大鼠淋巴细胞和中性粒细胞的数量及平均荧光强度进行动态监测．结果：模型兔血清对供体的细胞毒性无降低，但供体大鼠胸腺和单个核细胞能与受体兔抗大鼠抗体长期共存；大鼠在注射抗体后的１０ｍｉｎ～２０ｍｉｎ内细胞的平均荧光强度迅速下降．结论：受体体内的抗供体抗体并不明显影响供体组织或细胞在受体中的存活，即受体和移植物相互适应；其机制可能是移植细胞表面的抗原抗体复合物脱落导致细胞表面相应抗原缺失．
OBJECTIVE: To induce “body fluid adaptation” to xenotransplantation and to study its mechanism. METHODS: Recipient rabbits were treated with various immunosuppressive methods, then the rats were transplanted into the bone marrow and thymus of rabbits. The cytotoxicity and indirect immunofluorescence staining were used to detect the tissue and cells in the recipient rabbit At the same time, fluorescently labeled goat anti-rat mononuclear cell antibody was directly injected into rats, and the number and average fluorescence intensity of lymphocytes and neutrophils in rats were dynamically monitored by flow cytometry. RESULTS: The rabbit model had no cytotoxic effect on the donor but the donor thymus and mononuclear cells could coexist with the rabbit anti-rat antibody for a long time. The average cell number Fluorescence intensity decreased rapidly. CONCLUSION: The anti-donor antibody in the recipient does not significantly affect the survival of the donor tissue or cells in the recipient, ie, the recipient and the graft are adapted to each other. The mechanism may be that shedding of the antigen-antibody complex on the surface of the transplant results in the cell The corresponding surface antigen is missing.