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为探讨聚合酶链反应（ＰＣＲ）检测间日疟原虫（Ｐ．ｖ．）的现场应用价值。根据Ｐ．ｖ．红内期ＳＳｕｒＲＮＡ基因序列合成１对寡核苷酸引物建立检测Ｐ．ｖ．的ＰＣＲ技术，扩增产物片段为３４１ｂｐ，同时采用ＰＣＲ法和镜检法对７８份临床疑为Ｐ．ｖ．感染患者的血液标本进行检测。用ＰＣＲ法检测阳性者６９份，镜检法检测阳性者５９份，ＰＣＲ检出率（８８．５％）明显高于镜检法检出率（７５．６％），两者符合率为８７．２％。结果表明ＰＣＲ检测Ｐ．ｖ．具有简便、快速、特异性强、敏感性高等优点，可作为Ｐ．ｖ．诊断和流行病学调查的重要手段
To investigate the field application of polymerase chain reaction (PCR) in the detection of P. vivax (P.v.). According to P. v. One pair of oligonucleotide primers was synthesized to detect P in the red internal SSurRNA gene sequence. v. Of the PCR technology, the amplified fragment was 341bp, using PCR and microscopy of 78 clinical suspects p. v. Blood samples from infected patients were tested. 69 positive samples were detected by PCR and 59 were positive by microscopy. The detection rate of PCR (88.5%) was significantly higher than that of microscopy (75.6%), the coincidence rate was 87 .2%. The results showed that PCR detected P. v. Has the advantages of simple, fast, strong specificity, high sensitivity, can be used as P. v. Diagnostic and epidemiological survey of important means