论文部分内容阅读
目的研究p53蛋白和血管内皮生长因子(VEGF)在膀胱癌组织中的表达及其在膀胱癌诊断、分级及预后方面的意义。方法取88例膀胱癌患者癌组织切片,以免疫组织化学法测定其p53及VEGF表达水平,并比较其在首发与复发患者、WHO病理不同分级及TNM不同分期的患者组织中的表达情况。结果正常膀胱上皮组织中无p53及VEGF的表达,膀胱癌组织中p53及VEGF均为阳性染色,阳性率分别为76.1%和71.6%,两者在膀胱癌组织中的表达呈正相关(r=0.475,P<0.05);复发的患者p53、VEGF阳性率比首发患者高,在分化不好的G2、G3级患者中阳性率比分化良好的G1级高,在浸润性T2~T4期患者中阳性率比非浸润性Tis~T1期患者高,差异均有统计学意义(P<0.05)。结论 p53和VEGF对膀胱癌的诊断、病理分级、临床分期及复发可能的预后判断具有重要意义。
Objective To investigate the expression of p53 protein and vascular endothelial growth factor (VEGF) in bladder cancer and its significance in the diagnosis, classification and prognosis of bladder cancer. Methods Eighty - eight cases of bladder cancer were obtained. The expression of p53 and VEGF were detected by immunohistochemical method. The expression of p53 and VEGF in the tissues of the patients with primary and recurrent disease, WHO grading and TNM stage were compared. Results There was no p53 or VEGF expression in normal bladder epithelial tissues. The positive staining rates of p53 and VEGF in bladder cancer tissues were 76.1% and 71.6%, respectively. There was a positive correlation between the two expression levels in bladder cancer tissues (r = 0.475 , P <0.05). The positive rates of p53 and VEGF in relapsed patients were higher than those in the first patients, and the positive rates in patients with poorly differentiated G2 and G3 were higher than that in well-differentiated patients with G1, and positive in patients with invasive T2 ~ T4 The rates were higher than those in noninvasive Tis ~ T1 patients (P <0.05). Conclusions The expression of p53 and VEGF is of great significance in diagnosis of bladder cancer, pathological grade, clinical stage and prognosis of recurrent bladder cancer.