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发光免疫测定法(Luminescence Immu-noassay,LIA)是通过发光反应检测抗原抗体反应的一类分析方法。一般的发光反应是氧化-还原反应,在反应中能量以可见光的形式释放,可用发光计(Luminometer)或合适的仪器测定光发射率。这类发光测定法的灵敏度高,但特异性差。将灵敏度高的化学发光或生物发光反应与特异性高的抗原-抗体反应的免疫测定法相结合,就产生发光免疫测定法。发光免疫测定法是一种超微量的分析法,能分析10~(-12)～10~(-15)mol 浓度的生物样品,应用于医药、生物和生化的微量物质的测定中。在发光免疫测定中,在抗原或抗体上标记
Luminescence Immu-noassay (LIA) is a type of analysis method that detects the reaction of antigen and antibody by luminescence reaction. The general luminescent reaction is an oxidation-reduction reaction, in which energy is released in the form of visible light, and the light emissivity can be measured with a luminometer or a suitable instrument. Such luminescence assay has high sensitivity but poor specificity. Luminescence immunoassays are generated by the combination of highly sensitive chemiluminescent or bioluminescent reactions with highly specific antigen-antibody reactions. Luminescence immunoassay is an ultra-trace analysis method capable of analyzing biological samples in the concentration range of 10 ~ (-12) ~ 10 ~ (-15) mol for the determination of trace substances in medicine, biology and biochemistry. In luminescent immunoassays, the antigen or antibody is labeled