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目的观察莲房原花青素和银杏内酯联用对东莨菪碱所致小鼠学习记忆获得性障碍的改善作用。方法采用Morris水迷宫筛选出50只空间学习记忆能力相近的昆明种雄性小鼠,随机分为5组,即正常对照组、模型组、低剂量治疗组、中剂量治疗组、高剂量治疗组,每组10只。行为学测试前10min腹腔注射东莨菪碱,建立小鼠记忆获得障碍模型。3个治疗组分别灌胃50mg/kg莲房原花青素+10mg/kg银杏内酯、100mg/kg莲房原花青素+20mg/kg银杏内酯、150mg/kg莲房原花青素+30mg/kg银杏内酯;正常组和模型组以生理盐水代替。喂养30天后以Morris水迷宫法、跳台法行为学实验,观察莲房原花青素和银杏内酯的联同作用。结果在东莨菪碱所致小鼠记忆障碍模型中,莲房原花青素和银杏内酯联用可缩短在Morris水迷宫中的潜伏期,减少游泳距离。在跳台实验中莲房原花青素和银杏内酯联用可以延长平台停留期,显著减少错误次数。结论莲房原花青素和银杏内酯联用能够改善东莨菪碱所致小鼠学习记忆障碍。
Objective To observe the effect of lotus root procyanidins combined with ginkgolides on the improvement of learning and memory impairment induced by scopolamine in mice. Methods A total of 50 Kunming male mice with similar spatial learning and memory abilities were selected using the Morris water maze and randomly divided into five groups: normal control group, model group, low-dose treatment group, middle-dose treatment group, and high-dose treatment group. 10 in each group. Scopolamine was intraperitoneally injected 10 minutes before the behavioral test to establish a model of memory impairment in mice. The three treatment groups were intragastrically administered 50 mg/kg lotus procyanidins + 10 mg/kg ginkgo lactone, 100 mg/kg lotus procyanidins + 20 mg/kg ginkgolide, 150 mg/kg lotus procyanidins + 30 mg/kg ginkgolide; The group and model group were replaced with saline. After 30 days of feeding, Morris water maze test and step-down ethology experiment were conducted to observe the synergistic effects of lotus seed procyanidins and ginkgolides. RESULTS: In the scopolamine-induced mouse model of memory impairment, the combination of lotus seed procyanidins and ginkgo lactone shortens the incubation period in the Morris water maze and reduces swimming distance. In the step-down experiment, lotus seed proanthocyanidins combined with ginkgo lactone can extend the platform residence time and significantly reduce the number of errors. Conclusion The combination of lotus seed procyanidins and ginkgo lactone can improve scopolamine-induced learning and memory impairment in mice.