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HBV-DNA斑点杂交是一项敏感、特异的检测方法,我们主要参照北医附属人民医院肝病室学习班材料,结合自己的条件,建立了简化的方法,并用于临床。现将检测结果与其他血清学指标的比较报告如下。 在152例HBsAg阳性标本中,有127例HBV-DNA阳性(约占8.31％),其中34例HBeAg阳性标本HBV-DNA全部阳性,说明HBeAg确是病毒积极复制的一个标志。在9例抗HBe阳性血清中,也有6例HBV-DNA阳性;在103例抗HBc阳性血清中,有91例HBV-DNA阳性。HBV-DNA的检出率和HBsAg的滴度有关,一般情况下,滴度越
HBV-DNA dot blot hybridization is a sensitive and specific detection method, we mainly refer to the North Medical Affiliated Hospital of Liver Room learning class materials, combined with their own conditions, the establishment of a simplified method and used in clinical. Now test results and other serological indicators of the comparison are reported below. In 152 cases of HBsAg positive samples, 127 cases of HBV-DNA positive (about 8.31%), of which 34 cases of HBeAg-positive specimens HBV-DNA were all positive, indicating that HBeAg indeed a sign of positive viral replication. Of 9 anti-HBe positive sera, 6 were HBV-DNA positive; among 103 anti-HBc-positive sera, 91 were HBV-DNA positive. The detection rate of HBV-DNA and HBsAg titer, under normal circumstances, the more titers