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首次报告应用家族性腺瘤性息肉病(FAP)基因相关DNA探针估测无症状者患该病的危险性。共对七个FAP家系41倒有该病危险的无症状者(年龄0～39岁)进行研究。每例均以六种DNA探针检测基因型。先将从外周血白细胞提纯的DNA用适当的限制性内切酶进行消化,经琼脂糖凝胶电泳切割成特定片段,再以放射标记DNA探针杂交。然后在-70℃24～48小时以放射自显影显示补充这个探针的
The first report of the use of familial adenomatous polyposis (FAP) gene-associated DNA probes estimated the risk of asymptomatic persons suffering from the disease. A total of seven asymptomatic individuals (ages 0-39 years) with FAP families who were at risk for the disease were studied. In each case, six DNA probes were used to detect genotypes. The DNA purified from peripheral blood leukocytes is first digested with appropriate restriction enzymes, cut into specific fragments by agarose gel electrophoresis, and hybridized with radiolabeled DNA probes. Then autoradiograph display supplemented with this probe at -70°C for 24-48 hours