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目的 :观察金雀异黄素 (genistein)对体外培养的血管内皮细胞 (ECV 3 0 4)生长的影响和对过氧化氢引起ECV 3 0 4细胞损伤的保护作用。方法 :将ECV 3 0 4细胞与金雀异黄素 0 .1～ 1 0 0 μmol·L-1共同孵育 2 4～ 96h ,通过MTT法测定细胞存活率。为了观察金雀异黄素对过氧化氢引起ECV 3 0 4细胞损伤的保护作用 ,将细胞分成 3组 ,分别为对照组、过氧化氢损伤模型组和金雀异黄素治疗组 ,治疗组ECV 3 0 4细胞先与金雀异黄素 6.2 5～ 1 0 0 μmol·L-1共同孵育 3 0min ,然后模型组和治疗组ECV3 0 4细胞均加入过氧化氢 1 0mmol·L-1孵育 ,3 0min后以MTT法测定OD值 ,以此反映各组ECV 3 0 4的生存情况。结果 :金雀异黄素对于正常ECV 3 0 4细胞生长的作用与浓度和作用时间有关 ,浓度为1 0 0 μmol·L-1时 ,作用 2 4,48h能明显促进ECV3 0 4生长 (P <0 .0 1 ) ,而作用 96h则表现为明显的抑制作用 (P <0 .0 5 ) ;浓度为 1 0 μmol·L-1时 ,作用48,72h对ECV 3 0 4细胞生长也有促进作用 (P <0 .0 5 ) ;而当金雀异黄素浓度降低为 0 .1 ,1 μmol·L-1时 ,对ECV 3 0 4细胞生长无明显影响。过氧化氢 1 0mmol·L-1可使ECV 3 0 4的存活率明显下降(P <0 .0 5 ) ;预先给予金雀异黄素 2 5 ,5 0 ,1 0 0 μmol·L-1能浓度依赖性地
Objective: To observe the effects of genistein on the growth of cultured human vascular endothelial cells (ECV-304) and the protective effect of hydrogen peroxide on ECV-304 cells. Methods: ECV 3 0 4 cells were co-incubated with genistein from 0.1-1.00 μmol·L-1 for 2-496 h, and cell viability was measured by MTT assay. In order to observe the protective effect of genistein on hydrogen peroxide-induced ECV-304 cell injury, the cells were divided into 3 groups: control group, hydrogen peroxide injury model group and genistein treatment group, treatment group ECV304 cells were incubated with genistein at a dose of 6.2 5 ~ 100 μmol·L-1 for 30 min before ECV304 cells were incubated with 10 mmol·L -1 hydrogen peroxide , And the OD values were measured by MTT method after 30 minutes to reflect the survival of ECV304 in each group. Results: The effect of genistein on the growth of normal ECV-304 cells was related to the concentration and action time. When the concentration of 100 000 μmol·L-1, the effect of genistein on the growth of ECV-304 was significant <0.01), while the effect was obvious at 96h (P <0.05). At the concentration of 10 μmol·L-1 for 48 and 72h, the growth of ECV-304 cells was also promoted (P <0.05). However, when genistein concentration was reduced to 0.1 and 1 μmol·L-1, there was no significant effect on ECV-304 cell growth. Hydrogen peroxide (10 mmol·L -1) significantly decreased the viability of ECV304 (P <0.05). Pretreatment with genistein 25, 50, 100 μmol·L -1 Can be concentration-dependent