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本文主要介绍生物素—抗生物素蛋白系统的建立方法,以及用于固相免疫技术检测人体微量活性物质 IgE 的实验研究。整个检测系统由以下几个方面所组成:生物素的活化(BNHS 的制备)、生物素对抗体的标记、抗生物素蛋白-辣根过氧化物酶复合物的制备以及免疫检测的实验方法等。该系统用于固相免疫酶联技术,具有极高的灵敏度(0.1ng/ml)和特异性。
This article mainly introduces the establishment of biotin-avidin system, as well as the experimental study of solid phase immunosorbent assay (IgE) for human body trace active substances. The entire detection system consists of the following aspects: activation of biotin (preparation of BNHS), labeling of biotin-conjugated antibodies, preparation of avidin-horseradish peroxidase complex, and experimental methods of immunoassay . The system is used for solid-phase immunosensor technology with very high sensitivity (0.1 ng / ml) and specificity.