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目的 探讨呼吸道病毒感染与激素敏感型单纯性肾病综合征 (SSSNS)触发的相关性。方法 采用反转录—聚合酶链反应 (RT -PCR)、桥联酶标法 (APAAP)和酶联免疫吸附试验 (ELISA) ,检测 2 6例SSSNS患儿外周血单个核细胞 (PBMC)呼吸道病毒基因、病毒抗原和血清病毒抗体 ,并以正常儿童作对照。结果 ⑴SSSNS患儿活动期组PBMC中呼吸道病毒基因、病毒抗原及血清病毒抗体检出率明显高于缓解期组及正常对照组 (P <0 0 5 )。⑵SSSNS患儿病毒检出率与尿蛋白定量密切相关 (P <0 0 5 ) ,2 4h尿蛋白定量大于 5 0mg·kg- 1·d- 1)者 ,病毒检出率高于 2 4h尿蛋白定量小于 5 0mg·kg- 1·d- 1者 ,尿蛋白转阴时 ,体内病毒消失。结论 呼吸道病毒感染可能参与了SSSNS的发病
Objective To investigate the correlation between respiratory virus infection and hormone-sensitive simple nephrotic syndrome (SSSNS) triggering. Methods The respiratory tract of peripheral blood mononuclear cells (PBMCs) of 26 children with SSSNS were detected by reverse transcription-polymerase chain reaction (RT-PCR), enzyme linked immunosorbent assay (APAAP) and enzyme-linked immunosorbent assay (ELISA) Virus gene, virus antigen and serum virus antibody, and normal children as a control. Results (1) The detection rate of respiratory tract virus gene, virus antigen and serum virus antibody in PBMC of active group was significantly higher than that of remission group and normal control group (P <0.05). (2) The detection rate of virus in SSSNS patients was closely related to urinary protein (P <0.05), 24 h urinary protein was more than 50 mg · kg-1 · d-1, the detection rate of virus was higher than that of 24 h urine protein Quantitative less than 50mg · kg-1 · d-1, urine protein negative, the virus disappeared. Conclusions Respiratory virus infection may be involved in the pathogenesis of SSSNS