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本研究旨在筛选适宜花生上胚轴愈伤组织诱导的最佳培养基,建立并优化花生上胚轴愈伤组织诱导体系。实验选用不同类型的花生品种,以上胚轴为外植体,在添加有3~6 mg/L Pic和5~10 mg/L2,4-D的MS-B5诱导培养基上对上胚轴愈伤组织诱导。比较不同预培养时间的愈伤组织诱导差异,不同基因型诱导差异以及不同激素配比诱导差异。结果表明,预培养0天的珍珠豆型花生‘白沙1016’诱导率最高,确定MS+B5+Pic 3 mg/L为最适宜诱导培养基,MS+Pic 3 mg/L+Gln 1 g/L为继代培养基。该体系适宜本试验中多数花生品种的愈伤诱导,并可进行多次继代培养得到重复性体胚发生培养物。
The aim of this study was to screen the optimal medium for the induction of callus from the epicotyls of peanut and to establish and optimize the induction system of the epicotyl callus. Different types of peanut varieties were selected for experiment, and the upper hypocotyls were used as explants. The hypocotyls were induced on MS-B5 induction medium supplemented with 3 ~ 6 mg / L Pic and 5 ~ 10 mg / L 2,4-D Wound tissue induction. Differences in callus induction, differences in induction of different genotypes and differences in induction of different hormone ratios were compared between different pre-culture periods. The results showed that the induction rate of ’Baisha 1016’ was the highest in peanut peanuts pre-cultured for 0 days. MS + B5 + Pic 3 mg / L was the most suitable induction medium, MS + Pic 3 mg / L + Gln 1 g / L To subculture medium. The system is suitable for the callus induction of most peanut varieties in this experiment, and can be repeatedly subcultured to obtain repetitive somatic embryogenic culture.