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选择进入本研究的共有120例,所有受试者中无高血压、冠心病、脑血管病病史,其中单纯2型糖尿病(non-di-abetic retinopathy,NDR组)者31例,2型糖尿病合并背景期DR(background-diabetic retinopathy,BDR组)者30例,2型糖尿病合并增殖期DR(proliferative-diabetic retinopathy,PDR组)者29例及正常对照组30例。测定受试者禁食8～10小时过夜后的空腹血糖(FPG)、糖化血红蛋白(HbA1c)、TC、TG、HDL-C、LDL-C并测量身高、体重,计算BMI。将血标本按3000r/min离心10min后取血清,置于-20℃冰箱保存,采用酶联免疫吸附实验(ELISA)测定TNF-α的水平。结论 BDR组血清TNF-α水平明显高于NDR组,PDR组血清TNF-α水平高于BDR组,随着DM病程的延长及视网膜受损程度与TNF-α呈显著正相关。
A total of 120 patients were enrolled in this study. All subjects had no history of hypertension, coronary heart disease and cerebrovascular disease. Among them, 31 patients with NDR group and 31 patients with type 2 diabetes mellitus Thirty patients with background-diabetic retinopathy (BDR), 29 patients with type 2 diabetes mellitus with proliferative-diabetic retinopathy (PDR) and 30 patients with normal control group were included. Fasting plasma glucose (FPG), HbA1c, TC, TG, HDL-C and LDL-C were measured after fasting for 8 to 10 hours. Height and weight were measured to calculate BMI. The blood samples were centrifuged at 3000r / min for 10min and serum was taken and stored in a refrigerator at -20 ℃. The level of TNF-α was determined by enzyme-linked immunosorbent assay (ELISA). Conclusions The level of serum TNF-α in BDR group is significantly higher than that in NDR group. The level of serum TNF-α in PDR group is higher than that in BDR group. With the prolongation of DM course and the degree of retinal damage, the level of TNF-α is positively correlated.