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方法:将90只雄性小鼠随机分为A组、B组及C组,各30只。B组与C组均注射链脲佐菌素,制作备用糖尿病小鼠模型。B组苦瓜汤灌胃,C组山药汤灌胃,分别于3周、6周及8周后采集三组小鼠血液及尿液样本,用酶联免疫吸附法检测小鼠肾脏中血管内皮生长因子表达水平,分析苦瓜与山药对链脲佐菌素诱导糖尿病模型小鼠血糖血脂的影响。结果:B组、C组小鼠实验3周、6周、8周后体质量明显低于A组小鼠,组间对比差异显著(P<0.05),且血糖水平明显高于A组,B组与C组血糖水平无明显差异(P>0.05);C组VEGF表达水平明显低于B组,提示山药药物干预,对糖尿病小鼠肾脏可起到有效的保护作用。
Methods: Ninety male mice were randomly divided into group A, group B and group C, with 30 rats in each group. Both streptozotocin and streptozotocin were injected into groups B and C to make a model of spared diabetes mice. Group B bitter melon soup was given orally, and group C was treated with gavage. Blood and urine samples of three groups of mice were collected at 3 weeks, 6 weeks and 8 weeks respectively. Endothelial growth in mice kidneys was detected by enzyme-linked immunosorbent assay. The level of factor expression was used to analyze the effects of balsam pear and yam on blood glucose and lipids in streptozotocin-induced diabetic mice. Results: The body weight of mice in group B and group C after 3 weeks, 6 weeks and 8 weeks was significantly lower than that in group A. There was a significant difference between groups (P<0.05), and the blood glucose level was significantly higher than that of group A. There was no significant difference in blood glucose levels between group C and group C (P>0.05). The expression level of VEGF in group C was significantly lower than that in group B, suggesting that the intervention of yam medicine can effectively protect the kidney of diabetic mice.