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目的 探讨新生儿感染时外周血中性粒细胞 (PMN)功能异常的膜分子机制。方法 应用DPH荧光探针标记PMN膜 ,荧光偏振法测定 5 1例感染新生儿 (其中败血症 2 8例、肺炎 2 3例 )、19例健康新生儿PMN膜流动性。结果 感染新生儿PMN流动性较对照组比较显著降低 (P <0 0 1) ,尤以败血症更为明显 ;相关分析结果表明 ,PMN膜微粘度 (η)与吞噬、杀菌率呈负相关 ,相关系数 (γ)分别为 - 0 30 1(P <0 0 5 )、- 0 2 5 6 (P <0 0 5 )。结论 PMN膜流动性降低是造成感染新生儿PMN吞噬、杀菌功能降低的重要原因。
Objective To investigate the molecular mechanism of membrane dysfunction in peripheral blood neutrophils (PMN) during neonatal infection. Methods DPN fluorescent probe was used to label PMN membrane. Fluorescence polarization was used to determine the mobility of PMN in 51 neonates (28 in sepsis and 23 in pneumonia) and 19 healthy neonates. Results The fluidity of PMN infection in neonates was significantly lower than that in control group (P <0.01), especially in sepsis. Correlation analysis showed that there was a negative correlation between PMN microviscosity (η) and phagocytosis and bactericidal rate The coefficients (γ) were -0301 (P <0 05) and -0256 (P <0 05), respectively. Conclusion The decrease of PMN membrane fluidity is an important reason for the phagocytosis and bactericidal function of neonatal PMN.