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以7050B保持系(抗病)、TX622B保持系(感病)杂交的F2抗性个体和甜高粱丝黑穗病8113(抗病)、甜高粱丝黑穗病8101(感病)为试材,应用RAPD筛选技术对甜高粱丝黑穗病感、抗病基因组进行分子标记的初步分析.实验过程中采用CTAB法提取高粱、甜高粱基因组总DNA,应用琼脂糖凝胶电泳法对RAPD扩增结果进行检测,通过比较琼脂糖凝胶电泳电压的不同,进一步优化了琼脂糖电泳电压,以7050B保持系(抗病)、TX622B保持系(感病)杂交的子一代抗性个体为试材筛选了90个引物,其中29引物扩增出了多态性谱带,应用20个具有多态性扩增谱带的引物对甜高粱丝黑穗病8113(抗病)、甜高粱丝黑穗病8101(感病)进行了RAPD分析,结果表明共有9个引物扩增出了差异谱带,引物分别为S83,S-84,S-88,S-89,S-90,S-92,S-96,S-97,S-98.
F2 resistant individuals crossed with 7050B maintainer line (resistant), TX622B maintainer line (susceptible), sweet sorghum smut 8113 (resistant to disease) and sweet sorghum smut 8101 (susceptible) RAPD screening technique was used to analyze the molecular marker of sweet sorghum wire smut and disease-resistant genome.The total DNA of sorghum and sweet sorghum genomes was extracted by CTAB during the experiment, and the results of RAPD amplification by agarose gel electrophoresis The electrophoresis voltage of agarose was further optimized by comparing the different agarose gel electrophoresis voltages. The progenies of F1 generation with 7050B maintainer line (resistance) and TX622B maintainer line (susceptible) were screened Ninety-nine primers were used to amplify the polymorphic bands. Polymorphic bands were amplified by using 20 primers with 20 polymorphic bands. The results showed that there were 8113 sweet sorghum smut (disease resistance), 860 (Susceptible) were analyzed by RAPD. The results showed that a total of 9 primers amplified the difference bands. The primers were S83, S-84, S-88, S-89, S-90, S-92, 96, S-97, S-98.