论文部分内容阅读
通过ＥＢ病毒ＬＭＰ２Ａ重组痘苗病毒转染的ＤＣＳ体外诱导ＬＭＰ２Ａ特异性ＣＴＬ，并通过ＧＭ－ＣＳＦ、ＩＬ－４和ＴＮＦ－ａ培养体系，我们诱导出了人外周血单核细胞来源的ＤＣ。同时选用在鼻咽癌患者中表达的ＥＢ病毒潜伏蛋白之一ＬＭＰ２Ａ作为靶基因，利用重组痘苗病毒
We induced LMP2A-specific CTLs in vitro transfected with the recombinant vaccinia virus of LMP2A by Epstein-Bar virus and induced DCs derived from human peripheral blood mononuclear cells by GM-CSF, IL-4 and TNF-a culture systems. At the same time, LMP2A, one of EBV latent proteins expressed in NPC patients, was selected as the target gene. Recombinant vaccinia virus