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根际真菌是土壤生态系统的重要组成部分,本研究采用土壤盆栽方法,以纳米银(silver nanoparticles,Ag NPs)为研究对象,利用Illumina高通量测序技术对不同Ag NPs施加水平下(0.025、0.25、2.5mg/kg)潮土玉米根际真菌群落结构进行分析。结果表明,潮土玉米根际土壤真菌群落主要由子囊菌门Ascomycota、担子菌门Basidiomycota、芽枝菌门Blastodimycota、壶菌门Chytridiomycota、球囊菌门Glomeromycota和接合菌门Zygomycota等组成,其中以子囊菌门真菌为优势类群。Ag NPs在2.5mg/kg施加水平下显著降低了(P<0.05)玉米根际土壤溶解性有机碳(DOC)含量,改变了根际土壤真菌群落结构,使真菌群落结构发生显著分异(P<0.05),主要表现为降低了根霉菌属Rhizopus、镰刀菌属Fusarium、被孢霉属Mortierella等的相对丰度。相关性分析表明土壤DOC含量的变化与Ag NPs处理下根际土壤真菌群落结构分异存在显著的(P<0.05)相关性。
Rhizosphere fungi are an important part of soil ecosystem. In this study, soil potted plants were used to treat silver nanoparticles (Ag NPs) with Illumina high-throughput sequencing. 0.25, 2.5 mg / kg) rhizospheric soil fungi community structure analysis. The results showed that the fungal communities in rhizosphere soil of Chao soil were mainly composed of Ascomycota, Basidiomycota, Blastodimycota, Chytridiomycota, Glomeromycota and Zygomycota. Among them, ascus Fungal fungi as the dominant group. Ag NPs significantly decreased (P <0.05) the content of soil dissolved organic carbon (DOC) in the rhizospheric soil at 2.5 mg / kg level, changed the fungal community structure in rhizosphere so that the fungal community structure was significantly different (P <0.05), mainly showed that the relative abundance of Rhizopus, Fusarium, Mortierella and so on were reduced. Correlation analysis showed that there was a significant (P <0.05) correlation between changes of soil DOC content and rhizosphere soil fungal community structure under Ag NPs treatment.