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目的：探讨ＨＢＶ与肿瘤抑制基因ｐ５３相互作用的机制及其在原发性肝癌发生中的意义．方法：应用计算机程序对ＨＢＶ全基因组进行比较分析，合成含ＤＮＡ－蛋白质结合位点的基因片段作为探针，与肝癌细胞核蛋白作用．通过凝胶电泳移动试验，凝胶电泳超移动试验，原位紫外线交联试验，确定ＨＢＶ与ｐ５３蛋白的特异性结合．应用带报道基因ＣＡＴ与ｐ５３，ＨＢＶＸ基因共转染，观察ＨＢＶ与ｐ５３蛋白相互作用的生物学功能．结果：ＨＢＶ基因组增强子Ｉ上游区域（１０４７～１０５９）存在ｐ５３基因应答成份结合位点序列，能特异性地与ｐ５３蛋白结合，使ｐ５３蛋白在细胞内积聚．结论：ＨＢＶ与ｐ５３存在ＤＮＡ－蛋白质结合关系，在ＨＢＶ感染相关的肝癌发生中具有非常重要的理论意义．
Objective: To explore the mechanism of interaction between HBV and tumor suppressor gene p53 and its significance in the development of primary liver cancer. Methods: The whole genome of HBV was comparatively analyzed by computer program, and the gene fragment containing DNA-protein binding site was synthesized as a probe to act on the nuclear protein of hepatoma cells. The specific binding of HBV and p53 protein was determined by gel electrophoresis shift assay, gel electrophoresis ultra-mobile assay and in-situ UV cross-linking assay. Co-transfection with reporter gene CAT and p53 and HBVX gene was used to observe the biological function of HBV and p53 protein interaction. Results: The upstream of HBV genome enhancer I (1047 ~ 1059) had p53 gene response component binding site sequence, which could specifically bind with p53 protein and make p53 protein accumulate in cells. Conclusion: The DNA-protein binding relationship between HBV and p53 exists and has very important theoretical significance in the occurrence of hepatocellular carcinoma associated with HBV infection.