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株系紫云英品种的DNA为模板进行PCR扩增,筛选出40条扩增条带较好的ISSR引物,其中有10个引物扩增出的条带多态性较好,从500~3 000 bp共扩出684个条带,平均多态率59.2%,表明供试的紫云英品种资源的遗传多样性相依程度较高。采用NTSYS 2.1软件分析,22个紫云英品种间的相似性系数界于0.63~0.95。UPGMA法聚类将22个紫云英品种分为4类,研究表明ISSR分子标记方法可较好的应用于紫云英品种遗传多样性分析上,并为紫云英品种间的鉴定分类提供了分子水平的依据。
Strains of Astragalus species DNA as a template for PCR amplification, screening out 40 amplified bands better ISSR primers, of which 10 primers amplification of the band polymorphism is good, from 500 to 3 000 bp, a total of 684 bands were amplified, with an average polymorphism rate of 59.2%, which indicated that the genetic diversity of Astragalus species was highly dependent on the genetic diversity. Using NTSYS 2.1 software analysis, the similarity coefficients of 22 species of Astragalus sinicus ranged from 0.63 to 0.95. UPGMA clustering divides the 22 species of Astragalus sinicus into four groups. Studies have shown that the ISSR molecular marker method can be applied to the genetic diversity analysis of Astragalus species, and provides the identification classification Molecular level basis.