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本文报道应用核酸分子杂交方法,以[α-(32)P]-dTTP标记的HPV16型基因组为探针,共检测恶性肿瘤53例(标本包括宫颈癌22例,阴茎癌3例、膀胱癌6例、肾癌3例、肺癌7例、胃癌1例、结肠癌11例)、7例良性肿瘤、8例正常上皮组织中HPV基因组相关序列。检测结果表明,在恶性肿瘤中仅于宫颈癌组织检测出HPV16型基因组相关序列59.1％,P<0.01。而良性肿瘤和正常上皮组织中均未检测出HPV16型和其他型别基因组相关序列。此种阴性结果,可能与地理分布、病变组织的病理类型、检测使用的探针和方法、例数少等因素有关。为进一步探讨HPV感染与肿瘤的关系,尚需深入研究。
In this study, 53 cases of malignant tumors were detected by using HPV16 genotypes labeled with [α- (32) P] -dTTP by nucleic acid molecular hybridization (including 22 cases of cervical cancer, 3 cases of penile cancer and 6 cases of bladder cancer 3 cases of renal cell carcinoma, 7 cases of lung cancer, 1 case of gastric cancer and 11 cases of colon cancer), 7 cases of benign tumor and 8 cases of normal epithelial tissue. The results showed that only 59.1% of HPV16 genome-related sequences were detected in cervical cancer tissues, P <0.01. However, no relevant genotypes of HPV16 and other genotypes were detected in benign tumors and normal epithelium. Such negative results may be related to geographical distribution, the pathological type of diseased tissue, the probes and methods used for detection, and the small number of cases. In order to further explore the relationship between HPV infection and cancer, further study is still needed.