汉防己甲素及维拉帕米对成纤维细胞生长增殖的影响

• 作 者: 刘学松 李定国 陆汉明 徐芹芳
• 机 构: 上海医科大学病理教研室,上海第二医科大学附属新华医院消化研究室
• 出 处: 新消化病学杂志
• 发表日期: 1997年02期
• 关键词: 汉防己甲素 维拉帕米 成纤维细胞 生长增殖 抗肝纤维化 细胞蛋白质 细胞培养 抑制作用 细胞周期 药物疗法

目的探讨汉防己甲素（Ｔｅｔ）及维拉帕米（Ｖｅｒ）抗肝纤维化的机制．方法采用细胞培养、流式细胞术和图像分析法检测Ｔｅｔ及Ｖｅｒ对３Ｔ６成纤维细胞增殖的影响．结果不同浓度的Ｔｅｔ及Ｖｅｒ可使３Ｔ６细胞Ｇ１期或Ｇ２＋Ｍ期细胞显著增多（Ｐ＜００１）；Ｔｅｔ（１５，２０μｇ）使细胞ＲＮＡ含量显著下降（Ｐ＜００１）；Ｖｅｒ（１０，２０μｇ）使细胞ＤＮＡ含量显著降低（Ｐ＜００１）；Ｔｅｔ（１５，２０μｇ）和Ｖｅｒ（５，１０μｇ）均使细胞蛋白质含量显著增加（Ｐ＜００１）．结论Ｔｅｔ及Ｖｅｒ对成纤维细胞的增殖具有抑制作用，可能是抗肝纤维化的机制之一

Objective To investigate the anti-hepatic fibrosis mechanisms of tetrandrine (Tet) and verapamil (Ver). Methods The effects of Tet and Ver on the proliferation of 3T6 fibroblasts were detected by cell culture, flow cytometry and image analysis. Results Tet and Ver were significantly increased in G1 phase or G2 + M phase of 3T6 cells (P <001). Tet (15, 20 μg) significantly decreased cellular RNA (P <001) ; Ver (10, 20μg) significantly decreased the cellular DNA content (P <001); both Tet (15,20μg) and Ver (5,10μg) increased the cellular protein content significantly 01). Conclusion Tet and Ver have an inhibitory effect on the proliferation of fibroblasts and may be one of the mechanisms of anti-hepatic fibrosis