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体外淋巴细胞转化试验(以下简称淋转)是目前测定机体免疫状态的一种较好的方法,也是研究细胞免疫的常用手段。由于这一方法十分敏感易变,一旦操作不稳定,会严重影响到它的应用价值。本文似~3H-TdR掺入法淋转为例,简单综述了培养过程中诸细胞浓度对淋转的影响。一、淋巴细胞浓度对试验的影响 1、直接“接触抑制”用有丝分裂原(如PHA)刺激人淋巴细胞时,其DNA合成率随着淋巴细胞浓度的增加而上升,达到某一高峰然后下降,从细胞浓度反应曲线可见到DNA合成的最适
In vitro lymphocyte transformation test (hereinafter referred to as lymph node metastasis) is currently a measure of the immune status of a better method, but also a common means of research on cellular immunity. Because of this method is very sensitive and volatile, once the operation is not stable, it will seriously affect its value. This article like ~ 3H-TdR incorporation leaching as an example, a brief review of the culture process of the concentration of cells on the lymph node metastasis. First, the impact of lymphocyte concentration on the test 1, direct “contact inhibition” When using mitogen (such as PHA) to stimulate human lymphocytes, the DNA synthesis rate increases with the increase of lymphocyte concentration reached a certain peak and then decreased, From the cell concentration response curve can be seen DNA synthesis optimum