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目的:建立HPLC法测定健胃愈疡片中延胡索乙素的含量。方法:采用Agilent C18(250 mm×4.6 mm,5μm)色谱柱;流动相:甲醇-0.1%磷酸溶液(用三乙胺调pH至6.0)(60∶40),流速为1.0 ml.min-1,检测波长为280 nm,柱温30℃。结果:延胡索乙素在10.36～51.80μg.ml-1范围内线性关系良好(r=0.999 9),平均回收率为98.1%,RSD为1.3%(n=6)。结论:方法简便,结果准确,可用于控制健胃愈疡片的质量。
Objective: To establish an HPLC method for the determination of tetrahydropalmatine in Jianweiyuyang tablet. Methods: Agilent C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol-0.1% phosphoric acid solution (adjusted to pH 6.0 with triethylamine) (60:40) at a flow rate of 1.0 ml · min -1 The detection wavelength was 280 nm and the column temperature was 30 ℃. Results: Tetrahydropalmatine had good linearity (r = 0.999 9) in the range of 10.36 ~ 51.80μg.ml-1 with an average recovery of 98.1% and a RSD of 1.3% (n = 6). Conclusion: The method is simple and accurate and can be used to control the quality of Jianwei Yuyue tablets.