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目的:建立高效液相色谱法同时测定维药古丽娜中没食子酸、柯里拉京和鞣花酸的含量。方法:采用Venusil XBP C18色谱柱(4.6 mm×250 mm,5?m),以乙腈(A)和0.4%磷酸溶液(B)为流动相,梯度洗脱,流速1 m L·min-1,柱温25℃,检测波长254 nm,进样量10?L。结果:没食子酸,柯里拉京和鞣花酸的线性范围分别为0.585~9.360(r=0.999 7)、0.537~8.592(r=0.999 6)和0.393~6.288?g(r=0.999 0),平均回收率分别为100.8%(RSD=2.8%)、102.3%(RSD=2.0%)和98.16%(RSD=2.0%);不同产地中古丽娜药材没食子酸,柯里拉京和鞣花酸含量存在差异。结论:该方法简单快捷,重复性和稳定性较好,可用于维药古丽娜中没食子酸、柯里拉京和鞣花酸的测定,为古丽娜的质量控制提供参考。
Objective: To establish a HPLC method for the simultaneous determination of gallic acid, corilagin and ellagic acid in Gulina. METHODS: Venusil XBP C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase with gradient elution of acetonitrile (A) and 0.4% phosphoric acid solution (B). The flow rate was 1 m L · min- Column temperature 25 ℃, detection wavelength 254 nm, injection volume 10? L. Results: The linear ranges of gallic acid, corilagin and ellagic acid were 0.585 ~ 9.360 (r = 0.999 7), 0.537 ~ 8.592 (r = 0.999 6) and 0.393 ~ 6.288? G The recoveries were 100.8% (RSD = 2.8%), 102.3% (RSD = 2.0%) and 98.16% (RSD = 2.0%), respectively. The content of gallic acid, . Conclusion: The method is simple, rapid, reproducible and stable. It can be used for the determination of gallic acid, corilagin and ellagic acid in Gulina, which provides references for the quality control of Gulina.