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作为种传病原物，豌豆种传花叶病毒（ＰＳｂＭＶ） 必须能承受种胚的干燥脱水过程方能在胚细胞中存活并种传。为了研究ＰＳｂＭＶ承受种胚干燥脱水的机制，比较了该病毒在豌豆（ Ｐｉｓｕｍ ｓａｔｉｖｕｍ Ｌ．） 新鲜胚与干燥胚子叶细胞中的稳定性与分布方式。在新鲜的、未成熟胚的子叶细胞中，ＰＳｂＭＶ的外壳蛋白（ＣＰ） 受到部分降解，该病毒粒体及其ＣＰ在细胞质内呈环核分布。在干燥、成熟的胚的子叶细胞中，ＰＳｂＭＶ的外壳蛋白未受到任何降解，其粒体和ＣＰ不再呈环核分布，而是存在于位于细胞质边缘的多聚体中。免疫金标记电镜检查证明这类多聚体中含有ＰＳｂＭＶ的粒体。很明显，种胚的干燥脱水过程可改变ＰＳｂＭＶ在子叶细胞中的稳定性与分布方式，粒体多聚体的形成可能有助于ＰＳｂＭＶ在干燥脱水的胚细胞中的稳定与存活
As a pathogen, PSBMV must be able to withstand the desiccation and desiccation process of germ cells and survive in germ cells. In order to study the mechanism of PSbMV undergoing embryo dry dehydration, the stability and distribution of the virus in leaf cells of fresh and dried embryo of pea (Pisum sativum L.) were compared. In fresh, immature embryonic cotyledon cells, the coat protein (CP) of PSbMV is partially degraded, and the nucleosome and CP are cyclically distributed in the cytoplasm. In the dry, mature embryonic cotyledon cells, the coat protein of PSbMV is not degraded. The mitochondria and CP are no longer distributed in the nucleus, but exist in multimers at the margins of the cytoplasm. Immunogold labeling Electron microscopy proved that these polymers contain PSbMV in the mitochondria. Obviously, the desiccation process of PSBMV can change the stability and distribution of PSbMV in cotyledon cells, and the formation of multimers could contribute to the stability and survival of PSbMV in desiccated and dehydrated embryonic cells