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目的 了解凋亡相关基因p53、bcl2、bax、Fas在鼻咽癌(NPC)中的表达及相互关系。方法 在67例NPC、15例癌旁组织中用免疫组化技术标记bcl2、p53、bax、Fas;用免疫双标记技术同时标记p53与bcl2;从中选取15例NPC用DNA原位杂交技术检测bcl2基因扩增情况。结果 ①67例NPC中p53蛋白阳性38例(567%),bcl2蛋白阳性48例(716%),bax蛋白阳性28例(418%),Fas蛋白阳性29例(433%);②NPC中,p53蛋白与bcl2蛋白的阳性率显著高于癌旁组织,而bax蛋白与Fas蛋白的阳性率却显著低于癌旁组织;③p53蛋白与bcl2蛋白阳性表达间呈正相关。④bcl2/bcl2同二聚体在NPC中占优势;p53蛋白表达在bcl2/bcl2同二聚体占优势的肿瘤中显著高于在bax/bax同二聚体占优势的肿瘤中。结论 ①p53、bcl2、bax、Fas在NPC中有较高的阳性表达率,与NPC的发生、发展关系密切;②NPC中p53与bcl2蛋白表达呈正相关,提示NPC中p53蛋白主要不是突变型,有异于其它肿瘤。③抑制细胞凋亡的bcl2/bcl2同二聚体在NPC中占优势,尤其是在泡状核细胞癌中,提示其与NPC的发生及分化有关。
Objective To investigate the expression of p53, bcl2, bax and Fas in nasopharyngeal carcinoma (NPC) and their correlations. Methods The bcl2, p53, bax and Fas were detected by immunohistochemistry in 67 cases of NPC and 15 cases of paracancerous tissues. P53 and bcl2 were also detected by double immunofluorescence staining. 15 cases of NPC were selected by DNA in situ hybridization to detect bcl2, Gene amplification. Results ① The p53 protein positive rate was 38 (567%) in 67 cases, 48 cases (716%) were bcl2 protein positive, 28 cases (418%) were bax protein positive and 29 cases (433%) were positive Fas protein. The positive rates of bcl2 and bcl2 were significantly higher than that of paracancer tissues, while the positive rates of bax and Fas were significantly lower than those of paracancer tissues. (3) There was a positive correlation between p53 and bcl2 protein expression. ④bcl2 / bcl2 homodimers predominate in NPC; p53 protein expression was significantly higher in tumors dominated by bcl2 / bcl2 homodimers than tumors predominated by homodimers in bax / bax. Conclusions①The positive expression rate of p53, bcl2, bax and Fas in NPC is higher than that in NPC, and there is a close relationship between the expression of p53 and bcl2 protein in NPC, which indicates that the p53 protein in NPC is not mainly mutant, In other tumors. ③ Inhibition of apoptosis of bcl2 / bcl2 homodimers predominance in NPC, especially in the somatic cell carcinoma, suggesting that the occurrence and differentiation of NPC.