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青花菜采后花蕾迅速衰老,叶绿素和蛋白质含量明显下降,超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性随衰老进程先升高而后下降,丙二醛(MDA)持续增加,细胞膜透性迅速增大。20mg/L6-苄基腺嘌呤(6-BA)处理明显延缓叶绿素和蛋白质的降解,提高SOD活性,对CAT激活尤为明显,抑制了Haber-Weiss反应,降低过氧化氢(H2O2)和羟基自由基(OH)含量,延缓膜脂过氧化作用,降低子MDA含量,减小膜渗漏,延缓花蕾衰老。20mg/L6-BA中加入02%苯甲酸钠(SBN),对6-BA产生的各种延衰效应均有增效作用,进一步推迟花蕾衰老。200mg/L抗坏血酸(AsA)促进叶绿素和蛋白质降解,在提高SOD和CAT活性的同时,MDA反而明显增加,膜透性迅速增大,加速衰老。
The postharvest buds of broccoli senesce quickly, the contents of chlorophyll and protein decreased significantly, the activities of superoxide dismutase (SOD) and catalase (CAT) increased first and then decreased with the aging process, while the malondialdehyde (MDA) Cell membrane permeability increased rapidly. The treatment with 20mg / L 6-BA reduced the degradation of chlorophyll and protein obviously, increased the activity of SOD, especially the activation of CAT, inhibited the reaction of Haber-Weiss, reduced the content of hydrogen peroxide (H2O2) and hydroxyl radical (OH) content, delay membrane lipid peroxidation, reduce the content of MDA, reduce membrane leakage, delay bud senescence. The addition of 0.2% sodium benzoate (SBN) to 20 mg / L 6-BA has a synergistic effect on various delay effects induced by 6-BA, which further delayed flower bud senescence. Ascorbic acid (200mg / L) promoted the degradation of chlorophyll and protein. While increasing the activities of SOD and CAT, MDA increased but the membrane permeability increased rapidly and accelerated aging.