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采用电脉冲法，转化大分子复合物ＩｇＧ－荧光素进入小麦部分酶解（１２０ｍｉｎ）愈伤组织的细胞即半原生质体中，在荧光显微镜下观察发现一些细胞内有荧光发生，由此确定的较适宜脉冲转化条件如电场强度为２２５０－３０５０Ｖ／ｃｍ应用于转化外源基因ｐＺＦＸ２到小麦半原生质体，随后将其按愈伤组织的培养过程进行培养和抗ｋａｎａｍｙｃｉｎ筛选后获得成活愈伤细胞团块，存活率为１１．３％－１８．９％，这都说明已成功地将这两种物质转入了小麦细胞内，从而在小麦中可以绕过原生质体，以半原生质体作为外源基因的另一类受体系统将可能成为行之有效的转基因途径
Using electric pulse method, the macromolecule complex IgG-fluorescein was transformed into the semi-protoplasts of the partially digested wheat (120min) calli. Fluorescence was observed in some cells under fluorescence microscope, More suitable for pulse conversion conditions such as electric field intensity of 2250-3050V / cm applied to the transformation of foreign gene pZFX2 wheat semi protoplasts, and then they were cultured according to the callus culture and anti-kanamycin screening survival callus obtained Lumps with a survival rate of 11.3% -18.9%, all of which indicate that these two substances have been successfully transferred into wheat cells so that protoplasts can be bypassed in wheat and semi-protoplasts as exogenous Another type of receptor gene system will likely become an effective transgenic approach