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目的探讨实验性偏头痛大鼠痛觉超敏行为及颈髓C1、C2后角环氧化酶2(COX-2)在偏头痛大鼠中枢敏化过程中的表达。方法健康成年SD大鼠30只,随机均分五组:空白对照(A)组、假手术(B)组、生理盐水(C)组、新型致炎剂(NIS)4d(D)组和NIS 7d(E)组。用Von-Frey纤维丝测定各组动物眶周痛觉阈值,免疫组化、Western blot检测大鼠颈髓C1、C2后角COX-2表达的变化。结果 C组大鼠的疼痛行为与A组相仿(P>0.05)。E组大鼠建模后第4天眶周痛觉阈值明显低于A组(P<0.05)。D组、E组COX-2阳性细胞计数均高于C组(P<0.01)。E组大鼠颈髓后角COX-2蛋白表达量明显高于A组(P<0.05)。结论 NIS硬脑膜给药能有效诱导偏头痛大鼠产生痛觉超敏,并与大鼠颈髓后角COX-2表达的增加密切相关。
Objective To investigate the hyperalgesia behavior of experimental migraine rats and the expression of COX-2 and C1, C2 in the migraine rat during central sensitization. Methods Thirty healthy adult SD rats were randomly divided into five groups: control group (A), sham operation group (B), saline group (C), NIS 4d 7d (E) group. The periorbital pain threshold of each group was measured by Von-Frey fiber. The expression of COX-2 in the C1 and C2 posterior horn of cervical spinal cord was detected by immunohistochemistry and Western blot. Results The pain behavior in group C was similar to that in group A (P> 0.05). The threshold of periorbital pain was significantly lower in group E than that in group A on the 4th day after modeling (P <0.05). The counts of COX-2 positive cells in group D and group E were higher than those in group C (P <0.01). The expression of COX-2 protein in cervical spinal cord of E group was significantly higher than that of A group (P <0.05). Conclusion NIS dural administration can effectively induce hyperalgesia in migraine rats and is closely related to the increase of COX-2 expression in rat spinal cord.