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目的研究复方丹参明目(FDM)对糖尿病性视网膜病变大鼠的影响及作用机制。方法采用大鼠单次尾静脉注射链脲佐菌素(45 mg·kg~(-1))的方法制备糖尿病模型,继续普通饲料喂养14周,再制备成糖尿病性视网膜病变模型。雄性SD大鼠随机分为正常组,模型组,FDM低、中、高剂量组(200,400,800 mg·kg~(-1)),连续灌胃给药2个月。采用酶联免疫法(ELISA)检测血清中血管内皮生长因子(VEGF)、基质金属蛋白酶-2/9(MMP-2/9)、血小板衍生生长因子A/B(PDGF-A/B)、碱性成纤维细胞生长因子(bFGF)和胰岛素样生长因子1(IGF-1)的含量;采用免疫组化法检测视网膜组织中VEGF和血管内皮细胞生长因子受体2(VEGFR-2)的表达;HE染色观察糖尿病性视网膜病变大鼠视网膜病理学形态。结果与正常组比较,模型组大鼠血清VEGF、MMP-2/9、PDGF-A/B、bFGF和IGF-1含量均显著升高(P<0.01),视网膜组织中VEGF、VEGFR-2表达均显著增多(P<0.01)。与模型组比较,FDM中、高剂量组大鼠血清MMP-2/9、PDGF-A、bFGF和IGF-1含量均显著降低(P<0.05,P<0.01),其中FDM高剂量组的VEGF含量亦显著降低(P<0.01);FDM中、高剂量组大鼠视网膜组织中VEGF、VEGFR-2表达量均显著减少(P<0.01)。结论 FDM能显著改善糖尿病大鼠的视网膜病变,可能与通过抑制VEGF/VEGFR-2及其他促血管生成因子的表达有关。
Objective To study the effects of compound Danshen (Mesangia head eye) (FDM) on diabetic retinopathy in rats and its mechanism. Methods Diabetic rats were induced by a single intravenous injection of streptozotocin (45 mg · kg -1) into the tail vein of rats, and then fed with normal diet for 14 weeks. The diabetic retinopathy model was further established. Male Sprague-Dawley rats were randomly divided into normal group, model group, FDM low, medium and high dose groups (200, 400, 800 mg · kg -1) for 2 months. Serum levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2/9 (MMP-2/9), platelet-derived growth factor A / B (PDGF-A / B) (BFGF) and insulin-like growth factor 1 (IGF-1) were detected by immunohistochemistry. The expression of VEGF and VEGFR-2 in retinal tissue were detected by immunohistochemistry. Retinal Pathology in Diabetic Retinopathy Rats with HE Staining. Results Compared with the normal group, the levels of VEGF, VEGF-2/9, PDGF-A / B, bFGF and IGF-1 in the model group were significantly increased (P <0.01) Were significantly increased (P <0.01). Compared with the model group, the levels of serum MMP-2/9, PDGF-A, bFGF and IGF-1 in the FDM high and medium dose groups were significantly decreased (P < (P <0.01). The expression of VEGF and VEGFR-2 in the medium and high dose FDM groups were significantly decreased (P <0.01). Conclusion FDM can significantly improve retinopathy in diabetic rats, which may be related to the inhibition of the expression of VEGF / VEGFR-2 and other proangiogenic factors.