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Calcitonin gene-related peptide(CGRP)has the effect of antiarrhyth-mic,expectally to ischemtc arrhythmia.But up to now,it’s electro-physiological mechanism is not known very clearly.In this study,theaction potential and resting membrane potential(RP)in rabbit my-ocardial were recorded with standard glass microelectrodes tech-nique,the electrophysiotogical effects of CGRP on normal and simu-lated ischemtc condition were observed.The results showed that:(1)In normal condition,after afflication of CGRP(the concentration ofCGRP was 1×10~(-8)M,CGRP was purchased from sigma chemicalCo),RP and action potential amplltude(APA)increased significantly(P<0.01),the action potential duration(APD_(30),APD_(50))were notchanged obviously,but the APD_(90)was shorted obviously(P<0.05).(2)In simulated ischemic condition,CGRP had the same effects onRP and APA as normal condition.It prolonged the APD_(30)and APD_(50)obviously(P<0.05)and shorted the APD_(90)signficantly.This indicat-ed that CGRP could reverse the abnormal changes caused by ischemi-a.These results demonstrated that CGRP had stabilizing and protec-tive effects on ischemical myocardial.
Calcitonin gene-related peptide (CGRP) has the effect of antiarrhythmic mic, expectally to ischemtc arrhythmia.But up to now, it’s electro-physiological mechanism is not known very clearly.In this study, the action potential and resting membrane potential (RP) in rabbit my-ocardial were recorded with standard glass microelectrodes tech-nique, the electrophysiological effects of CGRP on normal and simu-lated ischemtc condition were observed. The results showed that: (1) In normal condition, after afflication of CGRP (the concentration ofCGRP was 1 × 10 -8 M, CGRP was purchased from sigma chemicalCo, RP and action potential amplltude APA increased significantly (P <0.01), the action potential duration (APD_ (30), APD_ (50) (2) In simulated ischemic condition, CGRP had the same effects onRP and APA as normal condition. It prolonged the APD_ (30) and APD_ (30) 50) obviously (P <0.05) and shorted the APD_ (90) signficantly.This indicat-ed that CGRP could rever se the abnormal changes caused by ischemi-a.These results demonstrated that CGRP had stabilizing and protec-tive effects on ischemical myocardial.