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目的:探讨位于脂氧酶12(LOX12)基因编码区Arg261Gln单核苷酸多态与胃癌发病风险的关系。方法:用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测148例胃癌患者和148例无肿瘤正常对照人群的LOX12的基因型,并以Logistic回归模型计算各基因型与胃癌发病风险的关系。结果:LOX12 Arg261Gln等位基因频率在胃癌组中(0.544)高于正常组(0.443)。与Arg/Arg基因型携带者相比,Gln/Gln基因型携带者发生胃癌的风险增加(OR=2.26,95%CI=1.15~4.46,P=0.018),而杂合基因型Arg/Gln不增加胃癌发病风险(OR=1.37,95%CI=0.77~2.44,P=0.284)。结论:LOX12编码区Arg261Gln遗传变异可能是胃癌发病的重要遗传易感因素。
Objective: To investigate the relationship between the single nucleotide polymorphism of Arg261Gln located in the coding region of LOX12 gene and the risk of gastric cancer. Methods: The genotypes of LOX12 in 148 gastric cancer patients and 148 non-tumor normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Logistic regression models were used to calculate the genotype And the risk of gastric cancer. Results: The allele frequency of LOX12 Arg261Gln in gastric cancer group (0.544) was higher than that in normal group (0.443). Gln / Gln genotype carriers had a higher risk of developing gastric cancer than those with Arg / Arg genotype (OR = 2.26, 95% CI = 1.15-4.46, P = 0.018), while heterozygous genotypes Arg / Gln Increase the risk of gastric cancer (OR = 1.37, 95% CI = 0.77 ~ 2.44, P = 0.284). Conclusion: The genetic variation of Arg261Gln in LOX12 coding region may be an important genetic predisposing factor in the pathogenesis of gastric cancer.