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目的研究JNK在生后小鼠背皮毛囊周期中的表达规律及探讨其对毛囊周期的调控作用。方法选择生后不同时期(1、7、14、16、21、31 d)C57BL/6J小鼠54只,采用RT-PCR技术,检测JNK mRNA在小鼠背皮毛囊周期中的表达情况;采用Western blot和免疫组化技术,进一步检测JNK蛋白在毛囊周期中的表达规律。结果 RT-PCR检测结果表明,在毛囊周期中,JNK1在生长早期(生后7、31 d)表达最强,退化期(生后16 d)和静止期(生后21 d)维持较弱的表达;JNK2在各时相点均有中等强度表达。单因素方差分析显示,JNK1的表达水平在生长早期(0.99±0.02)与静止期(0.30±0.01)间存在显著差异(P<0.05),JNK2在生长早期(0.77±0.01)与退化期(0.97±0.03)间存在显著差异(P<0.05)。Western blot与RT-PCR检测结果一致。免疫组化结果显示,在毛囊生长期,JNK主要表达于毛母质和外根鞘;进入退化期,JNK表达于外根鞘和上皮索;静止期毛囊中无表达。结论 JNK在毛囊周期中呈动态表达模式,生长期JNK可能通过影响毛母质细胞的增殖和/或分化来调节毛囊生长;退化期JNK可能涉及毛囊细胞的凋亡过程。
Objective To study the expression of JNK in the hair follicle cycle of postnatal mice and to explore its regulation on the hair follicle cycle. Methods Fifty-four C57BL / 6J mice were selected at different time points after birth (1, 7, 14, 16, 21, 31 d). RT-PCR was used to detect the expression of JNK mRNA in the mouse dermal- Western blot and immunohistochemistry to further detect the expression of JNK protein in hair follicle cycle. Results RT-PCR results showed that expression of JNK1 was the strongest during the early growth stage (7 and 31 days after birth), and remained weak in the degenerative stage (16 days after birth) and stationary phase (21 days after birth) JNK2 expressed moderately at each time point. One-way analysis of variance showed that there was a significant difference in the expression of JNK1 between the early growth stage (0.99 ± 0.02) and quiescent stage (0.30 ± 0.01) (P <0.05) ± 0.03) (P <0.05). Western blot and RT-PCR test results. Immunohistochemical results showed that JNK mainly expressed in hairy maternal and outer root sheath during the period of hair follicle growth; while in degenerative stage, JNK expression was in outer root sheath and epithelial cord; there was no expression in resting hair follicles. Conclusions JNK is a dynamic expression pattern in hair follicle cycle. JNK may regulate the growth of hair follicles by affecting the proliferation and / or differentiation of hair follicle cells in the growth phase. JNK may be involved in the apoptosis of hair follicle cells in degeneration phase.