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[Objective] To determine the content of linarin in RADIX et RHIZOMA JATAMANSI VALERIANAE by RP-HPLC method.[Method] The HPLC conditions were as follows:Zorbax SB C18 column as separation column(150 mm×4.6 mm,5 μm),mobile phase of methanol-0.1% acetic acid(V:V=70:30),flow rate of 1.0 ml/min,column temperature of 30 ℃ and detective wavelength of 326 nm.[Result] The linear relationship between linarin concentration and peak area was good in the range of 0.010-0.080 μg,(r=0.999 7).The average recovery rate of linarin was 98.2% with RSD value of 1.78%(n=5).[Conclusion] The RP-HPLC method established in this study is favorable for the determination of linarin in Valeriana wallichii DC.,which would provide reference for the quality control of Valeriana wallichii DC..
[Objective] To determine the content of linarin in RADIX et RHIZOMA JATAMANSI VALERIANAE by RP-HPLC method. [Method] The HPLC conditions were as follows: Zorbax SB C18 column as separation column (150 mm × 4.6 mm, 5 μm), mobile phase of methanol-0.1% acetic acid (V: V = 70:30), flow rate of 1.0 ml / min, column temperature of 30 ° C and detective wavelength of 326 nm. [Result] The linear relationship between linarin concentration and peak area The average recovery rate of linarin was 98.2% with RSD value of 1.78% (n = 5). [Conclusion] The RP-HPLC method established in this study is favorable for the determination of linarin in Valeriana wallichii DC., which would provide reference for the quality control of Valeriana wallichii DC.