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对来源于腔节藻属海藻Coelarthrum boergesenii(采自中国南海西沙群岛)的一株海洋内生真菌Aspergillus fumigatus的发酵液进行化学成分的分离及鉴定。采用硅胶柱色谱、凝胶柱色谱和高压液相色谱等分离手段,从乙酸乙酯萃取部分中分离得到16个次级代谢产物(1~16),经核磁共振、质谱等现代波谱技术鉴定了它们的结构,分别为gliotoxin(1)、bis(dethio)bis(methylsulfanyl)gliotoxin(2)、fumiquinazoline C(3)、(-)-chaetomium(4)、verruculogen(5)、fumitremorgin C(6)、brevianamide F(7)、fumigaclavine C(8)、decanedioic acid,2-methylene(9)、decanedioic acid,2-methylene-,1-methyl ester(10)、2-undecenedioic acid(11)、2-undecenedioic acid,1-methyl ester(12)、helvolic acid(13)、di-2-ethyl hexyl phthalate(14)、physcion(15)、emodin(16),其中化合物9、10和12为新天然产物。
The chemical constituents of the fermentation broth of Aspergillus fumigatus, a marine endophyte from Coelarthrum boergesenii (collected from Xisha Islands, South China Sea), were isolated and identified. Sixteen secondary metabolites (1-16) were isolated from the ethyl acetate fraction by silica gel column chromatography, gel column chromatography and high pressure liquid chromatography. Their structures were identified by modern magnetic resonance spectroscopy and mass spectrometry Their structures were gliotoxin (1), bis (dethio) bis (methylsulfanyl) gliotoxin (2), fumiquinazoline C (3), (-) - chaetomium (4), verruculogen (5), fumitremorgin C brevianamide F (7), fumigaclavine C (8), decanedioic acid, 2-methylene (9), decanedioic acid, 2-methylene-, 1- methyl ester (10), 2-undecedioic acid , 1-methyl ester (12), helvolic acid (13), di-2-ethylhexyl phthalate (14), physcion (15) and emodin (16). Compounds 9, 10 and 12 are new natural products.