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为解决使用细胞培养法进行甲型肝炎病毒灭活试验,经实验证明NJ-3株甲型肝炎病毒可在人肝癌细胞PLC/PRF/5株中快速复制增殖。用免疫荧光法检测,在维持培养5天时即可观察到细胞内典型的HAV特异性荧光颗粒,维持培养10天,TCID_(50)达10~(5.5)。该培养系统适用于消毒学试验。
In order to solve the inactivation test of Hepatitis A virus by cell culture method, NJ-3 strains of Hepatitis A virus can be rapidly replicated and proliferated in human hepatoma cell line PLC / PRF / 5. Immunofluorescence assay showed that typical intracellular HAV-specific fluorescent particles were observed after 5 days of culture, and maintained for 10 days with a TCID50 of 10-5.5. The culture system is suitable for disinfection tests.